We use none foot print, feeder-free in vitro reprogramming method to convertsomatic cells into patient specificiPSCs. IxCell™–iPSCs can be utilized as a reliable, unlimited, and ethically unbiased source for simulating personalized disease. Because of their pluripotency, IxCell™–iPSCs can be differentiated into various types of tissue cells, such as Cardiomyocytes, Hepatocytes, and Neurons, etc. IxCell™–iPSCs,carrying the phenotype of human disease,might also be useful for disease diagnosis. IxCell can provide IxCell™–iPSCs as the best in vitro cell model for disease research, diagnosis and personalized cell therapy evaluation.
Identification of IxCell™–iPSCs:
Figure 1 Phase Contrast
Figure 2 A-D Immunofluorescence staining of pluripotent markers. A-D：Nanog，Oct4， Sox2 , SSEA4.
Figure 3 In vitro EB differentiation . A-C: ectoderm(β-tubulin III), mesoderm（SMA）and endoderm（AFP）
Figure 4 Flow Cytometry analysis of pluripotent markers . A-C: Nanog, Oct4 and SSEA4
Figure 5 Karyotype analysis
Figure 6 Clearance test of episomal vector